Invitrogen - Molecular Probes - Figure 8.115 - Schematic representation of real-time PCR with TaqMan primers

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Updated: April 5, 2005

Figure 8.115 Schematic representation of real-time PCR with TaqMan primers. In the intact TaqMan probe, energy is transferred (via FRET) from the short-wavelength fluorophore on one end (green circle) to the long-wavelength fluorophore on the other end (red circle), quenching the short-wavelength fluorescence. After hybridization, the probe is susceptible to degradation by the endonuclease activity of a processing Taq polymerase. Upon degradation, FRET is interrupted, increasing the fluorescence from the short-wavelength fluorophore and decreasing the fluorescence from the long-wavelength fluorophore.