Invitrogen - Molecular Probes - Figure 8.116 - Schematic representation of real-time PCR with Scorpion primers

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Updated: April 5, 2005

Figure 8.116 Schematic representation of real-time PCR with Scorpion primers. In the hairpin loop structure, the quencher (black circle) forms a nonfluorescent complex with the fluorophore (green circle). Upon extension of the amplicon, the Scorpion probe hybridizes to the newly formed complementary sequence, separating the fluorophore from the quencher and restoring the fluorescence.