Invitrogen - Molecular Probes - Figure 1.71 - Effect of protein conjugation on the absorption spectrum of tetramethylrhodamine

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Updated: April 5, 2005

Figure 1.71 Effect of protein conjugation on the absorption spectrum of tetramethylrhodamine. The absorption spectrum of tetramethylrhodamine conjugated to goat anti–mouse IgG antibody (TMR-GAM, T2762) shows an additional peak at about 520 nm when compared with the spectrum of the same concentration of the free dye (TMR). Partial unfolding of the protein in the presence of 4.8 M guanidine hydrochloride (TMR-GAM + GuHCl) results in a spectrum more similar to that of the free dye.